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prime script ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script ii 1st strand cdna synthesis kit
    Prime Script Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+ii+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc12281537-116-29-38
    Average 99 stars, based on 11022 article reviews
    prime script ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa
    Article Snippet: The shoot meristem tissue of 4-week-old Algonquin and Chuancao No.7 plants was collected, and total RNA was extracted using TRIzol-RT Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Total RNA was reverse transcribed into cDNA using the Prime Script II cDNA Synthesis Kit (Takara, Japan) following the manufacturer’s protocol. .. Subsequently, real-time PCR analysis was performed using Roche SYBR-green reagent (Roche) as the reporter dye on the Bio-Rad CFX Connect TM sequence detection system.

    Article Title: Huoxin pill prevents excessive inflammation and cardiac dysfunction following myocardial infarction by inhibiting adverse Wnt/β‑catenin signaling activation.
    Article Snippet: Background: Myocardial infarction (MI) is the most common cause of cardiac injury, resulting in widespread and irreversible damage to the heart.. The incidence of MI gives rise to the excessive production of inflammatory cytokines that further promotes myocardial dysfunction.. Wnt/β-catenin signaling pathway is adversely activated during MI and plays an important role in the modulation of the inflammatory response following tissue injury.

    Article Title: 17β-estradiol ameliorates LPS-induced acute lung injury via mediating mitochondrial biogenesis and function in rats.
    Article Snippet: Sepsis-induced acute lung injury (ALI), and its severe manifestation, acute respiratory distress syndrome (ARDS), are complex conditions associated with high global mortality.. 17β-estradiol (E2), a sex hormone, is implicated in ARDS pathophysiology, but its mechanisms remain unclear.. In this study, we explored the role of E2 in sepsisinduced ALI using ovariectomized female Sprague-Dawley rats and A549 cells.

    Article Title: Circulating miR-141 as a potential biomarker for diagnosis, prognosis and therapeutic targets in gallbladder cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) from plasma, tissues and cultured cells. .. The reverse transcription reaction was carried out using the Prime Script II cDNA Synthesis Kit (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer’s protocol. ..

    Article Title: Circulating miR-141 as a potential biomarker for diagnosis, prognosis and therapeutic targets in gallbladder cancer.
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) from plasma, tissues and cultured cells. .. The reverse transcription reaction was carried out using the Prime Script II cDNA Synthesis Kit (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer’s protocol. ..

    cDNA Synthesis:

    Article Title: Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa
    Article Snippet: The shoot meristem tissue of 4-week-old Algonquin and Chuancao No.7 plants was collected, and total RNA was extracted using TRIzol-RT Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Total RNA was reverse transcribed into cDNA using the Prime Script II cDNA Synthesis Kit (Takara, Japan) following the manufacturer’s protocol. .. Subsequently, real-time PCR analysis was performed using Roche SYBR-green reagent (Roche) as the reporter dye on the Bio-Rad CFX Connect TM sequence detection system.

    Article Title: Huoxin pill prevents excessive inflammation and cardiac dysfunction following myocardial infarction by inhibiting adverse Wnt/β‑catenin signaling activation.
    Article Snippet: Background: Myocardial infarction (MI) is the most common cause of cardiac injury, resulting in widespread and irreversible damage to the heart.. The incidence of MI gives rise to the excessive production of inflammatory cytokines that further promotes myocardial dysfunction.. Wnt/β-catenin signaling pathway is adversely activated during MI and plays an important role in the modulation of the inflammatory response following tissue injury.

    Article Title: 17β-estradiol ameliorates LPS-induced acute lung injury via mediating mitochondrial biogenesis and function in rats.
    Article Snippet: Sepsis-induced acute lung injury (ALI), and its severe manifestation, acute respiratory distress syndrome (ARDS), are complex conditions associated with high global mortality.. 17β-estradiol (E2), a sex hormone, is implicated in ARDS pathophysiology, but its mechanisms remain unclear.. In this study, we explored the role of E2 in sepsisinduced ALI using ovariectomized female Sprague-Dawley rats and A549 cells.

    Article Title: Circulating miR-141 as a potential biomarker for diagnosis, prognosis and therapeutic targets in gallbladder cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) from plasma, tissues and cultured cells. .. The reverse transcription reaction was carried out using the Prime Script II cDNA Synthesis Kit (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer’s protocol. ..

    Article Title: RNF220 promotes the proliferation of leukaemic cells and reduces the degradation of the Cyclin D1 protein through USP22.
    Article Snippet: Ring finger proteins contain a characteristic ring finger motif and perform a wide range of biological functions in living organisms.. These genes are abnormally expressed in many cancers.. We found that the expression level of Ring finger protein 220 (RNF220) was negatively correlated with the disease-free survival (DFS) and overall survival (OS) of acute myeloid leukaemia (AML) patients.

    Article Title: Circulating miR-141 as a potential biomarker for diagnosis, prognosis and therapeutic targets in gallbladder cancer.
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) from plasma, tissues and cultured cells. .. The reverse transcription reaction was carried out using the Prime Script II cDNA Synthesis Kit (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer’s protocol. ..

    Article Title: Development and application of monoclonal antibody-based dot-ELISA and colloidal gold immunochromatographic strip for rapid, specific, and sensitive detection of tomato brown rugose fruit virus.
    Article Snippet: Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus that has become a great concern to tomato production industry.. Due to the lack of resistant cultivars, precise detection of ToBRFV is essential to prevent the spread of ToBRFV.. In this study, we produced highly sensitive and specific monoclonal antibodies against ToBRFV and established dot-enzyme-linked immunosorbent assay (dot-ELISA) and colloidal gold immunochromatographic strip (CGICS)-based methods for ToBRFV detection.

    Isolation:

    Article Title: 17β-estradiol ameliorates LPS-induced acute lung injury via mediating mitochondrial biogenesis and function in rats.
    Article Snippet: Sepsis-induced acute lung injury (ALI), and its severe manifestation, acute respiratory distress syndrome (ARDS), are complex conditions associated with high global mortality.. 17β-estradiol (E2), a sex hormone, is implicated in ARDS pathophysiology, but its mechanisms remain unclear.. In this study, we explored the role of E2 in sepsisinduced ALI using ovariectomized female Sprague-Dawley rats and A549 cells.

    other:

    Article Title: Comprehensive Sampling and Detection Strategies for the Field Surveillance of Tomato Brown Rugose Fruit Virus
    Article Snippet: First-strand complementary DNA (cDNA) was synthesized from 1 μg of total RNA using Prime-ScriptTM II cDNA Synthesis Kit (TaKaRa, Kusatsu, Shiga, Japan).

    Synthesized:

    Article Title: Development and application of monoclonal antibody-based dot-ELISA and colloidal gold immunochromatographic strip for rapid, specific, and sensitive detection of tomato brown rugose fruit virus.
    Article Snippet: Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus that has become a great concern to tomato production industry.. Due to the lack of resistant cultivars, precise detection of ToBRFV is essential to prevent the spread of ToBRFV.. In this study, we produced highly sensitive and specific monoclonal antibodies against ToBRFV and established dot-enzyme-linked immunosorbent assay (dot-ELISA) and colloidal gold immunochromatographic strip (CGICS)-based methods for ToBRFV detection.



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